Shipping Estimate
USA
- USA
- CAN
- USA
- CAN
Ships within 48 hours · Estimated delivery Jul 7 - Jul 12
Promo Codes Available:
For Your Every Summer RSVP, with Code: SUMMER15
Description
Human TRAM1 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be ultrasonically disrupted or repeatedly frozen and thawed. Finally, the homogenate is centrifuged at 5000×g for 5-10 minutes and the supernatant is collected for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
|||||||||||||||||||||||||||||||||
| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against Translocation Associated Membrane Protein 1 (TRAM1). After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of Translocation Associated Membrane Protein 1 (TRAM1) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Translocation Associated Membrane Protein 1 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
|
|||||||||||||||||||||||||||||||||
| Background | Translocation-associated membrane protein 1, also known as TRAM1, is encoded by the TRAM1 gene. It participates in the translocation of nascent protein chains into and through the endoplasmic reticulum (ER) membrane by promoting correct chain positioning within the SEC61 channel. It regulates the exposure of nascent secretory protein chains to the cytosol during translocation into the ER. It may affect the phospholipid bilayer near the lateral gate of the SEC61 channel, thereby promoting ER protein trafficking. Throughout the integration process into the ER membrane, it closely associates with the transmembrane (TM) domain of nascent membrane proteins. It associates with the second TM domain of nascent chains of the G protein-coupled receptor opsin/OPSD in the ER membrane, potentially facilitating membrane integration. Under ER stress conditions, it participates in the disposal of misfolded ER membrane proteins during the unfolded protein response (UPR), an integrated stress response (ISR) pathway, by selectively translocating them from the ER to the cytoplasm where they are located, leading to proteasome ubiquitination and degradation. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
|||||||||||||||||||||||||||||||||
| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates and other biological fluids |
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy
4.2 ★★★★★
Based on 351 reviews
Sort
Product Reviews
★★★★★ 1
Small
These run very small.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on December 21, 2025
★★★★★ 5
If you haven't switched to boxer briefs, you're missing out :)
Size: 11 Plus, Color: Microfiber - Colors May Vary
I figured I ought to provide a review, something I'm not prone to doing even though I often/always bank my purchases on item reviews, since I just bought me a second set of these underwear because they're just that good!
I bought my 1st set of these 2 months ago because I was on the hunt for some new comfortable underwear and omfort was my main criteria. Wasn't aware that I was dealing with some health issues that was causing a fair bit of internal inflammation, just knew that I had had enough of basically everything I had at the time because they were old, ratty looking and even my all-time favorites had begun feeling too... something... not exactly too tight, yet they just weren't very comfortable anymore either and I didn't need that kind of extra stress in my life. But. I'm a barely-makin-it domestic violence survivor.
Sure, I'm super proud to have gotten as far as I have in this world (considering that post-separation psychological terrorism and torture continue to thus day, 5 years after I broke free) but, by rights and family-rearing, I should be living in a whole different world right now... which is my way of saying that money is extra, EXTRA tight in my house...and yet, I still have "caviar tastes", ifykwim. Lol. And, frankly, neither I nor anyone else should have to scrimp on comfortable, classy-feeling, fashionable clothing no matter how small our clothing budget may be.
I was spurred to check out boxer briefs for women after reading about a few different women's favorite brands in the comments on soneones IG post (that must have been in regards to underwear, but heck if I can remember the details). So, off I went to scout around on Amazon to see what this "boxer briefs" business was about... desperately hoping that the term was not just a rebranding of those "hipsters" things.
I am 46yo, 5'10" (potentially closer to 5'11"... not something I care enough about to know for certain, lol) and, clothing size-wise, my whole adult life I have consistently been a 16-18/18-20, top to bottom, with a shape that seems some what apple-ish at the moment but has, in general, always been more hourglass than apple. Also it seems as if I might have rather freakishly high hips, lol... not really, but for reference, when it comes to pants or jeans what generally passes as "tall", usually a 33"-34" inseem, fit like ankle or cigar length pants on me. But evidently I don't just have long legs, no, this momma's body seems to also have one heck of a long, tall pelvis which I did finally measure to figure out for sure but, prior to that, I could tell based on the way that low cut underwear like bikini & hipsters, and any form of low rise pants, barely make it north of my low lying c-section scar location which, for those who can't relate, is basically at the top of my hooha or just at the point where my belly and my hooha meet. And because that point on my body is not even remotely close to my "hip" area such items basically fall right off my body unless they're super stretchy and I deliberately hike them up to my hip area which I put up with in my 20s and 30s, for the sake of fashion I suppose, but which was and is quite ungodly uncomfortable, and... basically... I am so over with "uncomfortable" anything (men included, lol.)
In both cases I bought size 11, though, if I'm being honest, I am certain that a 10 would have fit well, too. And who knows, a 10 might have been just as comfortable or even more comfortable than the 11... but, I didn't take bother taking the chance to find out when I ordered my second set because the first set (size 11) made me so unbelievably happy that they literally changed my views about underwear for life.
This whole "pants come off after work" thing or the memes about people working from home (due to covid restrictions) and not wearing pants never jived with me prior to having these boxer briefs, but now... omg... these things are super inexpensive and somehow still suit my somewhat "caviar taste" perfectly (have a good handfeel and are just so unbelievably super comfortable), PLUS... they're opaque and they basically fit (and look) like I'm wearing a loungy pair of bike shorts, so... yeah, I've spent some time over the last 2 months actually chillin in my house in my freaking underwear!!!!
Life. Changing. :D
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on June 7, 2022
★★★★★ 5
Soft and comfy
Size: 12 Plus, Color: Microfiber - Colors May Vary
Comfortable fit. Have bought 4 times.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 30, 2026
★★★★★ 4
No elastic around the thighs is kinda nice!
Size: 9, Color: Cotton Stretch - Colors May Vary
Great undies! They fit good and the fabric is comfortable. Only thing is that the legs tend to ride up/bunch up with pants on. I got them for post surgery comfort and thats what I got.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 14, 2026
★★★★★ 5
Soooo comfortable
Size: 11 Plus, Color: Microfiber - Colors May Vary
Easy on/off because they really do stretch but return to shape. Fit is close to the body but not tight. Love that there is no elastic binding around my legs. Clothes glide over them because they are super smooth. Might never buy another style again.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 5, 2026