Shipping Estimate
USA
- USA
- CAN
- USA
- CAN
Ships within 48 hours · Estimated delivery Jul 6 - Jul 11
Promo Codes Available:
For Your Every Summer RSVP, with Code: SUMMER15
Description
Human RRP8 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. 4. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000 × g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash the collected cells three times with ice-cold PBS and resuspend them in 150-200 μL of PBS per 1 × 10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500 × g for 10 minutes at 2-8°C, and collect the supernatant for analysis. 5. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
|||||||||||||||||||||||||||||||||
| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against ribosomal RNA-processing protein 8 (RRP8). After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of ribosomal RNA-processing protein 8 (RRP8) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Ribosomal RNA-processing protein 8 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
|
|||||||||||||||||||||||||||||||||
| Background | Ribosomal RNA processing protein 8 is a protein encoded by the RRP8 gene. Diseases associated with RRP8 include essential tremor and spermatogenesis failure. 65 Its pathways include activating PKN1, stimulating transcription and chromatin regulation/acetylation of AR (androgen receptor)-regulated genes KLK2 and KLK3. Gene Ontology (GO) annotations associated with this gene include RNA binding and methylated histone binding. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
|||||||||||||||||||||||||||||||||
| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenates, cell lysates and other biological fluids |
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy
4.6 ★★★★★
Based on 638 reviews
Sort
Product Reviews
★★★★★ 5
Very helpful
Good plan to have.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on February 21, 2026
★★★★★ 5
Asurion worked as intended.
After three years, my monitor failed. Asurion paid to send it to a repair center. I didn't save the box the monitor came in, and paid $23 to have it packaged up. They kept me informed of the various steps, such as en route, arrived, testing, and repair started. They discovered it could not economically be repaired and refunded my purchase price. The entire process from me telling them it was broken until the refund took 5 days. I am well satisfied with the service.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on August 31, 2025
★★★★★ 5
A great phone...but compared to what?
Digital Storage Capacity: 128 GB, Color: Black
Tldr: Good phone for this price, noticeably better than older A13 and I wish more people would include context to their reviews. Glad I bought mine.
I'm leaving a long and detailed review because I got tired of reading through reviews that seemed contradictory, or said something was "bad" but without any context. If I made a poor choice buying a phone I'd likely be stuck with it for some years. I'm hoping this helps others like myself who are reading the reviews.
Ok, so the most important context is what phone I'm coming from over to the A17 5g. My old phone was Samsung A13 5g. My opinions of the new phone will be largely based on how they compare to the previous phone I used. So it's budget phone to budget phone, with the A17 having approximately 3-4ish years newer tech (or same tech but cheaper.) Both phones are being used on metro PCS (T mobile) network.
So I've now had the A17 for exactly one week. I primarily use my phone for android auto, text, checking email, web browser, occasional YouTube and maybe once per week on some games. I don't use it for heavy loads like massive file transfers or rendering video. It's fairly light use overall.
So far, the things I've been most pleased with are the screen and software/system layout. The screen has decent color and brightness and is MUCH better than the A13. Both screens claim to run at 90hz but the change from the old 270 PPI (I believe stands for pixel per inch or some equivalent) to 385 PPI feels massive. Much cleaner image from text to images. The change from LCD to AMOLED gives much better contrast with deeper colors. The software and system layout are a bit harder to describe in detail. It's more of a personal/ascetic preference. Things look and feel a lot cleaner. There are added optional features like side slide tabs to house shortcuts for certain apps. Another feature is top down swipe from the right for system shortcuts and from the left for notification, so they are smashed together.
Another improvement, though less so than the screen and software changes, is in the speed and responsiveness of the phone. This one also needs to be put in context a bit. I'm sure some would complain about it while coming from a more expensive and technologically superior phone. I have my own bias, as I'm coming from an inferior phone which had over 3 years of use (slowing it down over time.) I also use it for mostly light tasks. That being said, I am pleased that the phone is noticeably more responsive than my old one. Most of the lagging happens on start up (goes away after a few mins while the phone is connecting a bunch of apps and putting out/requesting info like date/time/location.) When I booted it for the first time, that was the worst lag I had. It will also slow down if you have several apps running at the same time. I feel like that's obvious, but some people genuinely don't know that if you hit the home button to leave an app, you need to bring up multi view and actually swipe the box away to close it fully. If you never close apps and never restart your phone on occasion it WILL slow way down. While using the phone for max 1 or 2 things at time it runs perfectly fine, and is better than the A13.
Battery life is good. I went from appx. 5000mAh to 5000mAh phones, so besides the slight degradation in the old phones battery life it should be the same. 5000mAh for my uses feels excellent overall. It can go for a full 2 days without charging. Charging speed itself seems improved, but that's never mattered too much for me so I can't comment much. Seems fine though, roughly 1.5h charge from mostly dead to full. That's using a 25w fast charger block and appropriate USB c cable. I didn't use the cable that came with the phone so I can't comment. It didn't come with a charger block, by design. Your mileage on charge speed will vary based on what you're using to charge the phone with. The phone can't charge at full speed if you're using a 5w charger from 15 years ago.
I don't have much to say about the camera. I rarely take photos and when I do it's usually quick snap shots of paper instructions or to share location. I believe (though I'm not sure) the A13 was also 50mp front camera but either the the other 2 lenses have been upgraded or they improved the camera software, because pictures do look a bit cleaner. It's not night and day or anything, but it looks better to my eye. Camera is also faster to adjust light and contrast when first focusing on a object, as compared to the old A13.
Build quality seems a little improved. They are both budget phones and how build quality "feels" is inherently subjective but to me it does seem a little better.
A quick note on activating this new phone...this may only be an issue I personally had, but in case this helps someone else I'd like to include it. I had a bit of trouble switching my physical SIM card out of my old phone and into the new. The metro app was telling me the phone wouldn't be compatible unless I changed phone plans (which is BS.) I had to call customer service and they opted to use the eSiM feature, rather than swapping the physical Sim. I noticed they also used the new phones 2nd SIM number to activate it, while i had tried using first. If you're having trouble perhaps check the phones info from the settings menu, and then try eSIM activation using the 2nd address. It worked fine for me after this.
Samsung has a quick transfer feature if your previous phone was a Samsung. It basically copies over everything from your old phone, including apps, pictures, contacts and even text message history ect. It took 3 or 4 attempts (it didn't want to connect and dropped the connection once at 30% complete.) Luckily if it disconnects part way through, it will pick up where it left off, without needing to restart. Once it finally had a stable connection it took me about 6 minutes, it will vary based on how much stuff you have to move over. You need to download an app to do the transfer, but it will prompt you and take you to it's link.
Summery: I find it pretty impressive how nice this phone feels both compared to its older model and for its price point in general. I found it frustrating that people would say this part or that was crap or great, but without giving any context to what they based that opinion off of. In my opinion, based on how cheap this phone is and compared to its older model (A13) this is an awesome phone so far, being one week in. Screen is a big improvement, system software and layout is better (IMO) and they seem to be making incremental improvements to things like the camera and overall build. For this price point I find it really impressive what you actually get. If you're coming from an older mid range phone I expect it will either be inferior or about the same. If you're coming from another phone in the 200 range it should be decent to good. For less than 200 and for what I use it for though, I'm very happy I bought this phone.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 3, 2026
★★★★★ 5
Great phone at a reasonable price
Digital Storage Capacity: 128 GB, Color: Gray, Digital Storage Capacity: 128 GB, Color: Gray
I purchased the Samsung Galaxy A17 5G for my mom, and it has been a great choice for her. She was able to set it up and start using it without any confusion, which was important to me. The screen is bright and clear, making it easy for her to read messages, browse the internet, and view photos. The camera takes nice pictures, and the battery lasts long enough that she doesn’t have to worry about charging it constantly. The fact that it was unlocked made it easy for her to use it with the carrier in the Bahamas. Overall, it’s a reliable, easy-to-use phone that offers excellent value for the price.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on June 7, 2026
★★★★★ 4
Good phone for the money
Digital Storage Capacity: 128 GB, Color: Blue
Got this for my wife after the battery in her Motorola phone blew up like a balloon. It's a decent phone. Camera is better than her old one. Glad that it has a SD card for her pics. Sometimes it's a bit laggy when she tries to unlock it. Works fine once it wakes up. All in all, I'd say it's a good value for the money for people who don't need a super high end phone.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 18, 2026
recommand products
Sterling Silver Gold-plated LogoArt Montana State University Bobcat Small Dangle Bead Charm
80.60
Sterling Silver Gold-plated LogoArt Michigan State University Letter S Dangle Ball Post Earrings
67.14
Bilstein B8 Performance Plus-Shock Absorber (24-131766)
154.00
Bilstein B4 OE Replacement-Shock Absorber (19-197692)
115.00
Bilstein B4 OE Replacement-Shock Absorber (24-188654)
89.00