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Description
Human TINF2 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be ultrasonically disrupted or repeatedly frozen and thawed. Finally, the homogenate is centrifuged at 5000×g for 5-10 minutes and the supernatant is collected for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a TERF1 Interacting Nuclear Factor 2 (TINF2) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of TERF1 Interacting Nuclear Factor 2 (TINF2) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human TERF1 Interacting Nuclear Factor 2 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | TERF1-interacting nuclear factor 2 (TINF2), also known as TRF1-interacting nuclear protein 2 (TIN2), is a protein encoded by the TINF2 gene. It is a component of the shelterin protein complex found at the ends of telomeres. This gene encodes one of the proteins of the shelterin or telosome complex, which protects telomeres by allowing cells to distinguish telomeres from areas of DNA damage. The protein encoded by this gene is a key component of shelterin. It interacts with the three DNA-binding proteins of the shelterin complex and is important for the assembly of the complex. Mutations in this gene cause dyskeratosis congenita (DKC), an inherited bone marrow failure syndrome. It has been shown to interact with ACD, POT1, and TERF1. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates and other biological fluids |
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4.3 ★★★★★
Based on 1263 reviews
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Product Reviews
★★★★★ 5
Color me impressed!
Color: Stainless Steel
Color me impressed! So far I really like this Typhur Sync oven. My first test was a frozen pizza. Unfortunately, it was overcooked. I really can't remember at this moment the exact temperature/time settings I used. (Probably used the box directions.) However, next time I'm going to just select the pizza setting on the oven and trust it. This is a convection oven, so I shouldn't have used the box directions. My next test was a big one. I cooked a 5.6 pound rib roast for Christmas. I selected “beef”, then “medium well”, then hit start. After the preheat was completed, I put the roast in the oven. Of course, before putting it in the oven I inserted the wireless temperature probe. I had taken the roast out of the fridge to let it get to room temperature an hour or so earlier. The probe said its internal temperature was 56 degrees, and the target temperature would be 140 degrees. I looked at other ovens as well, but selected this Typhur for its wireless probe and phone app that would track the oven progress and alert me when it was completed. The oven is downstairs, and this app would save me much stair work. It worked great! Love the little app. I checked the app from time to time for cooking progress, and it would tell me its current internal temperature and the target temperature. Also told me the time to completion. Amazing. It “dinged” my phone when the oven was done. I went downstairs and removed the roast. It looked fantastic. Of course while sitting on the counter top, the roast continued to cook internally while we were getting everything else set up on the dining table, thereby making it more like “well done” when we were finally ready to eat. After slicing it, the meat looked great. Not as pink as I wanted, but that was due to the “internal cooking” it did. I think next time, I'm selecting “medium”. Then it might be more like “medium well” when we're ready to eat. Also, did I mention that the cook time was just a little over 90 minutes? Couldn't believe how fast it cooked. My next test was cooking a pork tenderloin “medium well”. It took 33 minutes, and turned out great. Again, I'm very happy with this purchase. Back story... Years ago we remodeled our kitchen in a previous house, and not wanting to discard a perfectly good wall oven, I built a box for it and kept the oven in our garage to act as our second oven. Well, after 26 years it finally started failing. (Couldn't blame it for all the hot summer and cold winter cycles it went through.) Repair cost would be unreasonably high, even if the parts were available. Looking to replace it would cost anywhere between $450 (for a “no name” oven) and $1000 or more (for a “known brand” oven). I then saw these counter top ovens. After some study, I gambled and selected this Typhur Sync for $230 (also a “no name” brand to me at the time). Glad I did.
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Reviewed in the United States on December 28, 2025
★★★★★ 5
Good little oven
Color: Stainless Steel
Delivery was prompt. Wasn’t sure what to expect on the oven but the controls were fairly intuitive and have used several of the settings. Performs well on everything that we’ve used it for. The instructions are basic but adequate to get you cooking. The size was a little larger than anticipated, it is deeper and takes up more counterspace but you can cook a chicken in it! Looks great sitting on the counter. Not too industrial and the buttons and knobs make changing settings easy.
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Reviewed in the United States on January 19, 2026
★★★★★ 5
We love it!
Color: Stainless Steel, Color: Stainless Steel
Simply said, it’s great!
My wife absolutely loves this little oven. It has a bunch of options to choose from for cooking. You can use it as an air fryer, oven, you can cook perfect crispy wings, as well as a nice piece of steak with the included thermometer.
Since it’s very little compared to the regular house stove, its much easier and quicker to clean it.
It’s a must have for any home!
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Reviewed in the United States on February 15, 2026
★★★★★ 4
Best Toaster Oven Ever!
Color: Stainless Steel
I've had the oven about a week now and have used it numerous times. The air fried wings came out perfect. The oven baked chicken was perfectly cooked thanks to the probe and crisped up nicely. Toast is fast and just the right color each time. I give the oven high marks for the value and features. The oven's performance exceeds my expectations. The only reason I did not give this gem 5 stars is due to the oven door handle. When open, the door handle hits the counter top preventing it from opening fully. When the oven is hot, pulling something out is an issue due to the door not opening fully. Otherwise, I highly recommend this oven.
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Reviewed in the United States on December 26, 2025
★★★★★ 5
Great Product and Great Customer Service
Color: Stainless Steel
This little countertop oven checks all of the boxes for me.
First, even at its highest tempeture for baking bread at 450 the outside of the unit is warm to the touch but will not burn your hand.
It has all of the settings one would want for an oven to do icluding, reheat, PROOF (for bread) broil, stay warm etc. I love it and the best of all of this was my experience with their customer service. I started having an electrical glich with the unit soon after I bought it and reported the problem to the company. Well, then it stopped having that problem but intermittently did. The light would come on by itself and sometimes I could not get the oven to turn on. So, this ensued off and on for several months until I decided to once again contact customer service to report my problem. They took my information and then informed me that they would be happy to replace the unit. All I needed to do was send my unit back after receiving the replacement. Which they did and I did and VOILA, problem solved just like that. No rigamarole, no hassle just excellent customer service.
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Reviewed in the United States on May 1, 2026
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