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Description
Rat IL18-BP ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. 4. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 40 ng/mL). Then dilute to the following concentrations: 40 ng/mL, 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 40 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 20 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotinylated detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with Interleukin 18 Binding Protein (IL18-BP) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of Interleukin 18 Binding Protein (IL18-BP) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Rat | |||||||||||||||||||||||||||||||||
| Synonym | Rat Interleukin 18 Binding Protein ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Interleukin-18 binding protein (IL18BP) is a protein encoded by the IL18BP gene. This gene encodes an inhibitor of the proinflammatory cytokine IL-18. This protein binds to IL-18, preventing it from binding to its receptor, thereby inhibiting IL-18-induced IFN-gamma production. This protein is constitutively expressed and secreted in monocytes. Its expression can be enhanced by IFN-gamma. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.62-40 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenates and other biological fluids |
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4.4 ★★★★★
Based on 269 reviews
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Product Reviews
★★★★★ 5
Such a pretty bottle and it feels so great on skin!
Size: 1.7 Fl Oz (Pack of 1), Size: 1.7 Fl Oz (Pack of 1)
I was so excited to try this serum, and honestly, it did not disappoint. What first caught my eye was the packaging—I’m a sucker for pretty bottles, and this one is stunning with its shimmery, rainbow-like colors. Even the serum itself has a subtle shimmer that matches the bottle, which I absolutely love.
The scent was the first thing I noticed. It has a light floral smell with a hint of honey, almost like a soft version of the old Sweet Pea scent from Bath & Body Works. It’s not overpowering at all. You only really smell it when dispensing or applying it—once it’s on your skin, the scent completely disappears. No headaches, no lingering fragrance.
Application-wise, this serum is silky smooth and spreads beautifully. It made me realize my skin wasn’t as soft as I thought—because after using this, my skin felt noticeably softer, hydrated, and plump. It absorbs well and leaves my skin feeling nourished without being greasy.
It does contain retinol, so the warnings about avoiding the eye area and limiting sun exposure are important. I’ll definitely be pairing this with a good hydrating sunscreen, especially during daytime use. I plan to ease into it by using it once or twice a week until my skin fully adjusts.
Overall, I love everything about this serum—the packaging, the light scent, the texture, and the results. My skin feels smooth, hydrated, and healthy. This is definitely a product I’m excited to keep using and would happily recommend.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on January 11, 2026
★★★★★ 5
The best serum!
Size: 1 Fl Oz (Pack of 1)
This is the best face serum, and the price is unbeatable. It makes my face feel silky smooth and hydrated for the whole day. I will not use anything else.
Plus, it smells good. Highly recommend!
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Reviewed in the United States on May 27, 2026
★★★★★ 5
Smells great and silky smooth
Size: 1 Fl Oz (Pack of 1)
⭐️⭐️⭐️⭐️⭐️
I’ve been using the Olay Super Serum for a few weeks now and I’m honestly impressed with how much it does in one step. My skin looks noticeably brighter and more even, and it feels super hydrated without being greasy or heavy. It absorbs quickly, which I love because I can layer moisturizer and makeup right after without any pilling.
The texture is silky and lightweight, and a little goes a long way. I’ve also noticed my skin feels smoother and a bit firmer, especially around my cheeks. It gives a really nice healthy glow that looks natural, not shiny.
If I had to mention one small thing, it’s that you need to use sunscreen during the day since it contains AHA — but that’s expected with brightening products anyway.
Overall, this is a great all-in-one serum if you want brighter, smoother, more hydrated skin without using a bunch of separate products. I’d definitely repurchase and recommend it for anyone looking to simplify their skincare routine while still getting real results.
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Reviewed in the United States on March 2, 2026
★★★★★ 4
Yes
Size: 1.7 Fl Oz (Pack of 1)
Happy with this purchase
Very light on face , scent is light and fresh
Leaves my skin soft , hydrated and moisturized
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Reviewed in the United States on May 22, 2026
★★★★★ 5
Miraculous and so inexpensive!
Size: 1.7 Fl Oz (Pack of 1)
This stuff is amazing! I am getting benefits I never dreamed…I have only had it 3 days but had to report my immediate results!
I have a patch of seborrhea on my left eyelid that is an annoyance daily. I tried this serum on my eye and no burning or stinging. No irritation at all! I put my makeup on and thought little more of it. At the end of the day, no dry flaky patch or makeup issues there at all! No eye itching or irritation all day! This is something I thought I just had to live with.
As for the general serum I think the lines around my eyes are less prominent and my skin feels soft and moisturized.
And last I love the large bottle! Great value! And I am not embarrassed to have the bottle sitting on my sink…
Overall I think it is liquid gold for a bargain price! Will definitely buy again. Truly unexpected miracle in a bottle!
Highly recommend.
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Reviewed in the United States on January 22, 2026