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Description
Human H+/peptide transporter ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against Solute carrier family 15 (H+/peptide transporter), member 2 (HPEPT2). After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of Solute carrier family 15 (H+/peptide transporter), member 2 (HPEPT2) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Solute carrier family 15 , member 2 (HPEPT2) ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Hydrogen ion/peptide transporter 2 (HPEPT2), also known as solute carrier family 15 member 2, is an enzyme encoded by the SLC15A2 gene. It is a proton-coupled amino acid transporter that transports oligopeptides of 2 to 4 amino acids, with a preference for dipeptides. It transports the dipeptide-like aminopeptidase inhibitor bestatin, the aminocephalosporin antibiotic cefadroxil, and carnosine. It participates in innate immunity by facilitating the detection of microbial pathogens by NOD-like receptors. It may function by mediating the transport of bacterial peptidoglycans across the plasma membrane, catalyzing the transport of certain bacterial peptidoglycans, such as muramyl dipeptide (MDP), a NOD2 ligand. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.312-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.6 ★★★★★
Based on 2167 reviews
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Product Reviews
★★★★★ 5
Great product!
Style: 30 sqft
I can say this product makes a huge difference with road noise and is cleaner and easy to work with when applying compared to other mats that cost about the same price.
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Reviewed in the United States on February 14, 2026
★★★★★ 5
Very heavy and thick
Style: 30 sqft
I purchased mini brands of sound ending and this is by far the heaviest sound deadening I've ever ordered. I'm not installed yet but I have a good feeling that is going to work exceptionally in blocking sound. If weight is a concern for you I would recommend a lighter product however if sound quality and a quiet ride is your priority this is the one for you.
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Reviewed in the United States on March 18, 2026
★★★★★ 5
Siless Hybrid 3-in-1 - Far Exceeded my Expectations!!!
Model: SLH20025
I recently installed the Siless Hybrid 3-in-1 sound deadening mat in my vehicle, and I’m genuinely impressed. For just $80 for 25 square feet, this product delivers performance that rivals materials costing twice as much — and with far less hassle. If you're on the fence......Just buy it! You will not be disappointed.
Easy to Install: Unlike traditional sound deadening products that are stiff, sharp, and temperature-sensitive, this mat is soft, flexible, and easy to work with. I cut it cleanly with regular scissors and pressed it into tight spaces using just my fingers, so if in you don't have a roller or install tool, a good pair of scissors and your hands can get the job done. With other brands like Dynamat, the aluminum layer often leaves micro cuts on your hands and tears up your fingertips, but Siless does not due the acoustic layer being on top and above the aluminum layer that holds the butyl layer. So, it's very easy on your hands.
Even in cooler temperatures (around 50°F), I had no trouble applying it but it goes on better heated. If warm outside, I would leave the entire box out in the sun for at least 30 minutes when it's warm or heats it's very pliable and easy to mold. Make sure its where you want it because once applied it's not ever falling off. and it became even more pliable and easy to mold. No heat gun, no mess — just straightforward application.
I installed 40–50 square feet throughout the interior, covering everything except the headliner and the flooring forward of the rear seat. The difference in cabin noise is astonishing. I can turn my radio up concert loud with gut-punching bass, and from outside the car, it sounds like a factory sound system at mid volume. I was blown away by how well it contains sound — both inside out and outside in.
My exhaust is pretty aggressive and now is barely audible from inside the cabin. While driving, it almost sounds like a stock factory setup. The Siless matting actually changed the driving experience and feels refined.
If you're skeptical about sound deadening gimmicks, I get it, but this is the first product I've used and when I needed more, I went Stright to the 3 in 1 Hybrid matting and purchased it again. I love researching before buying and that's very uncommon for me to do.
Note: Proper installation is key, and it does take time and patience. But if installed correctly and in the right places, it works better previously used from paint-on sound deadening to Dynamat.
Update Nov, 2025
After using it this in 4 separate vehicles now. I feel the Siless’s 3 in 1 Hybrid has nailed it for best budget deadener that is better than dynamat extreme in performance and installation
Protip: buy a roll of 2-3” aluminum tape and tape the seams. After trimming the edges tend to very sticky between the panels. I ran a strip of aluminum tape over the seems to create a better seal and true moisture
I used it on four vehicles now and have I have not been paid to review and purchased every order myself.
3 in 1 Hybrid deadener by Siless is the best all around easy to apply sound deadner period!
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Reviewed in the United States on September 29, 2025
★★★★★ 5
Top quality
Model: SLH20025
This Siless Hybrid 3-in-1 sound deadening mat is easy to work with and feels high-quality. The butyl material is thick and pliable, making it simple to cut and fit into doors, floors, or panels.
After installation, road noise and vibrations are noticeably reduced, and it also seems to improve the overall cabin feel. No bad smell.
At 25 sq ft per roll, it’s a good value for anyone looking to dampen noise and improve car acoustics. Overall, a solid, effective option for automotive soundproofing. Used on a Fiesta ST.
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Reviewed in the United States on February 15, 2026
★★★★★ 5
Made a big improvement in cabin noise—highly recommend
Model: SLH20025
Siless Hybrid made a huge difference in reducing noise in the floor of my truck cabin. In some areas, there was basically just bare sheet metal between the inside and outside, so adding this really helped the truck feel more solid and noticeably quieter on the road.
I used Kilmat on the doors, and honestly I wish I had used Siless Hybrid there too, but it was too late by the time I realized the difference. This material is very sticky, easy to cut, and simple to apply, which made the install pretty smooth.
Just a heads-up: the tacky side gets messy and will bond to clothing really easily, so be careful during installation. Overall, I’d definitely buy it again—great product with real results.
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Reviewed in the United States on December 2, 2025
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